Cryo-Electron Microscopy of FVIII bound to Phospholipid Vesicles
نویسندگان
چکیده
Membrane-binding of a human blood clotting protein, factor VIII (FVIII), was studied by cryoelectron microscopy. Well visible gap junctions between adjacent phosphatydyl serine containing phospholipid vesicles were observed upon FVIII association. Digital images acquired with a JEOL2010FEG transmission electron microscope equipped with a 4x4k CCD camera were analysed with the Digital Micrograph software to study the lipid protein interactions and shed light onto the mechanism of FVIII membrane-binding. This study has been carried out in conjunction with ongoing structural electron microscopy studies of membrane-bound FVIII to better understand its role in blood coagulation and haemophilia.
منابع مشابه
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Factor VIII (FVIII) is a multidomain blood plasma glycoprotein. Activated FVIII acts as a cofactor to the serine protease factor IXa within the membrane-bound tenase complex assembled on the activated platelet surface. Defect or deficiency in FVIII causes haemophilia A, a severe hereditary bleeding disorder. Intravenous administration of plasma-derived FVIII or recombinant FVIII concentrates re...
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